Journal: medRxiv

Article Title: The angiotensin type 2 receptor agonist C21 restores respiratory function in COVID19 - a double-blind, randomized, placebo-controlled Phase 2 trial

doi: 10.1101/2021.01.26.21250511

Figure Lengend Snippet: Daily estimates of the proportion of patients in need for supplemental oxygen therapy. The left panel shows the total intention to treat population (C21 N=51, placebo N=55). The upper right panel shows patients in need for supplemental oxygen on the day of randomization (C21 N=37, placebo N=39), and the lower right panel shows patients not in need for oxygen on the day of randomization (C21 N=14, placebo N=16).

Article Snippet: In conclusion, C21 on top of standard of care, including glucocorticoids and remdesivir, significantly improved respiratory function reflected by a reduced need for supplemental oxygen in hospitalized COVID-19 patients.

Techniques:

Journal: Russian Journal of Physical Chemistry

Article Title: Effect of Et2Zn on Binding Spike Protein of SARS-Cov-2 and ACE2 from Cell Membrane

doi: 10.1134/S0036024423050163

Figure Lengend Snippet: Donor–acceptor results, E2 (resonance energy), occupancy, hybrid for RBD/Gly-Phe/hAP and RBD/Cys-Glu/hAPN

Article Snippet: RBD/Cys-Glu/hAPN , 0.8923 , BD (1) Zn9–C21 s 1 p 0.43 d 0.18 , BD (1) Zn9–C2 (Donor) BD*(1) C21–O22 (Acceptor) , 0.86.

Techniques:

Journal: Journal of Medicine and Life

Article Title: Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression

doi: 10.25122/jml-2022-0299

Figure Lengend Snippet: Renal tissue P-AKT staining scores of all groups measured by IHC; C21-treated group shows significantly improved P-AKT results compared to the sepsis control group (#P 0.05 vs. control).

Article Snippet: C21 was purchased from Medchem Express MCE in the USA and made in dimethyl sulfoxide (DMSO) as the standard vehicle before being diluted in N.S. at a ratio of 1:1 and prepared just before usage.

Techniques: Staining, Control

Journal: Cell reports

Article Title: AAV5-mediated manipulation of insulin expression in choroid plexus has long-term metabolic and behavioral consequences

doi: 10.1016/j.celrep.2023.112903

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal Rabbbit anti-TTR (prealbumin) AbCam Cat # ab75815; RRID:AB_1310604 Monoclonal Rabbit anti-Insulin Cell Signaling Cat # 3014; RRID:AB_2126503 Polyclonal Rabbit anti-mCherry Rockland Cat # 600-401-P16; RRID:AB_2614470 Monoclonal Rabbit anti- p -Akt (Ser473) Cell Signaling Cat # 4060; RRID:AB_2315049 Monoclonal Mouse anti-Alpha1 Na+/K+ ATPase AbCam Cat # ab7671; RRID:AB_306023 Bacterial and virus strains AAV5-CMV-CRE Vector Biolabs Cat # 7012 AAV5-CMV-GFP Vector Biolabs Cat # 7006 AAV5-CMV-Ins2 Vector Biolabs Cat # AAV-262269 AAV5-CMV-GCaMP6f Vector Biolabs Built to order AAV5-TTR-hM3Dq-mCherry Vector Biolabs Built to order AAV5-CMV-Psck1 Vector Biolabs Cat # AAV-268239 AAV5-CMV-Pcsk2 Vector Biolabs Cat # AAV-268242 Chemicals, peptides, and recombinant proteins Compound 21 TOCRIS Cat # 5548 Critical commercial assays PicoPure RNA Isolation Kit ThermoFisher Cat # KIT0204 Ultra Sensitive Insulin ELISA Kit CrystalChem Cat # 90080 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE218188 Experimental models: Cell lines iPS(IMR90)-4 WiCell WISCi004-B Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Strain # 000664 Ai14 The Jackson Laboratory Strain #007914 Ins1 −/− Ins2 fl/fl This paper N/A Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism GraphPad https://graphpad.com R R Foundation for Statistical Computing https://www.r-project.org Other Normal chow diet Dyets Inc Cat# 101845 High fat/high sugar diet Dyets Inc Cat# 103806 Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Plasmid Preparation, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Software